How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

How to measure the bonding strength of transfered graphene on PDMS substrate?

Chemical vapor deposition (CVD) was used to grow graphene on copper (Cu ) substrate, then the single layer graphene was transfered onto a PDMS substrate. I want to measure the bonding strength of...

17 October 2020 3,567 3 View

How to calculate bulk density, porosity, and void ratio of soil?

If the particle diameter is 2 mm and the particle density is 2.5 g/cm, what is the bulk density, porosity, and void ratio of this soil? I had known that the formula to calculate them. The...

11 October 2020 3,466 3 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View