We need help from people who have contracted mouse xenograft model with HCC1954 cell line. When 1x10e6 cells/mouse (50% matrigel) cells are scanned in a 6w female mouse, a tumor volume is formed about 100mm^3 after about 3 weeks. After that, the growth rate of cancer is jagged. The tumor tissue is not solid(hard), and the inside of the tumor continues to show symptoms of water filling. I tried about 5 times for animal efficacy experiments, but these problems still appear. I'm looking for advice if you've done efficacy experiments with this cell line.
Hi Seung-Hey,
I have no experience in the HCC1954 cell line, but the liquid-filled tumor texture seems necrosis. We used to come across some similar situations. We failed to establish xenografts (subQ model) in nude mice. Some tumors did not develop and some developed like what you described.
What strain did you use for the model? Some mice, even immunodeficient strains, can still eliminate the tumor cells. Probably you can perform a histology staining to check the xenografts to find out what's inside. Also, switching mouse strains to more immunodeficient ones may help.
Best,
YH