How can I convert Candida albicans OD600 absorbance to CFU/mL value?

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Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,796 3 View

How to calculate on-grid PV capacity of a factory?

Dear Researchers; I have a short question. Let's say a factory consumes 4 MW of electricity per hour. (fixed consumption throughout the year). I want to install an on-grid PV power plant for this...

29 January 2024 10,048 1 View

Candida albicans attached on the microplate's corners of the well?

I have a problem with broth dilution assay test for looking MIC of antimicrobial material. When I put my all staff(including antimicrobial materials) inside wells, then I put on my microplate on...

31 December 2023 9,269 2 View

How do you determine the bearing capacity by using Deflectometer (LFWD)?

Dear RG users, I am trying to determine the bearing capacity of road subbase/subgrade materials after compaction. We have a light falling weight deflectometer (LFWD) ready to use. Bear in mind...

04 September 2023 6,434 3 View

Antimicrobial material which is insoluble in water. When I dissolve in DMSO and diluted, it precipitated?

Antimicrobial material which is insoluble in water. When I dissolve in DMSO and diluted it with water(%10), it precipitated in the bottom of the tube. how can I use material for antimicrobial test?

20 August 2023 5,643 13 View

What is potency of powder?

MIC is calculate with this formula but how to calculate potency of powder? Weight of powder(mg)=Volume of Solvent (ml) X Concentration (mg/ml)/Potency of powder(ug/mg)

04 April 2023 6,094 2 View

How Can I get the stress-strain curve of loose materials?

Dear colleagues, How can I measure the unconfined compressive strength of the loose materials such as soils, to determine the stress and strain curve? Is there any other way to determien the...

30 April 2019 5,210 3 View

Do you have any research ideas on "static liquefaction" of soils?

Dear RG Users, I have gained an interest in studying static liquefaction phenomena, however, I constantly see some other terms. Are there any other names that are being used for it? Ex: flow...

31 March 2019 8,116 10 View

Which softwares are available for Material Point Method?

Dear RG users, I am looking to investigate the Material Point Method in Geotechnical applications. Do you know any available software for this purpose? It can be etiher commercial or open source....

01 February 2019 1,475 25 View

How can I determine the exact location of wedge and planar failures?

Hello RG users, I was wondering how can I determine the exact location of expected wedge, planar or toppling falures in rock slopes. Assume that I have already done kinematical analyses and...

13 December 2018 6,134 16 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,591 3 View

What is the most effective method for knocking out a gene in Candida albicans?

I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...

15 July 2024 3,977 1 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,419 3 View

Oral chicken Candida infection?

What you know about oral chicken Candida infection?

31 May 2024 9,040 1 View

Is it okay to compare the different result of different organisms in Minimum Inhibitory Concentration? or not since they're not the same?

The organism we used are Saccharomyces cerevisiae and Candida tropicalis. In the disk diffusion Candida tropicalis produce inhibition with 5mm but in Saccharomyces cerevisiae has 0 inhibition. But...

30 May 2024 1,349 0 View

Is this type of observation in checkerboard assay common?

Dear esteemed researchers, I've conducted some checkerboard assays to determine the fractional inhibitory concentration on several Candida sp. However, I've been getting some odd readings...

04 May 2024 3,408 0 View

I found a yeast in Kombucha. But not sure about the BLAST result?

Hello everyone. I am working on antimicrobial properties of yeast isolated from kombucha. So I chose 2 (out of 4) yeast strains with the highest antimicrobial properties, ran a PCR test and then...

24 February 2024 7,420 5 View

Alterations in the color of C. albicans colonies induced by the presence of calcofluor white?

Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?

30 January 2024 2,215 1 View

Candida albicans attached on the microplate's corners of the well?

I have a problem with broth dilution assay test for looking MIC of antimicrobial material. When I put my all staff(including antimicrobial materials) inside wells, then I put on my microplate on...

31 December 2023 9,269 2 View

When we required crisper over homologous recombination ?

I am happily using homologous recombination for candida genetic experiments then suddenly a question came in my mind why some people use crisper and other methods for gentic methods and major...

15 November 2023 2,935 0 View