Bekir Mustafa Yoğurtçu

7 Questions 6 Answers 0 Followers

Questions related from Bekir Mustafa Yoğurtçu

There is an abnormal curve in the real-time PCR. What could be the reason?

I am performing real-time PCR using cDNA (50 ng), primers (0.2 µM), and SYBR Green Master Mix. However, the reaction and cycle curve I obtained are as shown below. What could be the reason for...

03 February 2025 7,646 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

06 August 2024 10,633 3 View

Candida albicans attached on the microplate's corners of the well?

I have a problem with broth dilution assay test for looking MIC of antimicrobial material. When I put my all staff(including antimicrobial materials) inside wells, then I put on my microplate on...

01 January 2024 9,385 2 View

How can I convert Candida albicans OD600 absorbance to CFU/mL value?

How can I convert Candida albicans OD600 absorbance to CFU/mL value

21 September 2023 8,350 3 View

Antimicrobial material which is insoluble in water. When I dissolve in DMSO and diluted, it precipitated?

Antimicrobial material which is insoluble in water. When I dissolve in DMSO and diluted it with water(%10), it precipitated in the bottom of the tube. how can I use material for antimicrobial test?

21 August 2023 5,769 13 View

What is potency of powder?

MIC is calculate with this formula but how to calculate potency of powder? Weight of powder(mg)=Volume of Solvent (ml) X Concentration (mg/ml)/Potency of powder(ug/mg)

05 April 2023 6,222 2 View

How can I clean low density polyethylene with antimicrobial powder from a twin screw extruder?

Cleaning polymer from extruder screw

04 March 2015 7,255 10 View