Antimicrobial material which is insoluble in water. When I dissolve in DMSO and diluted it with water(%10), it precipitated in the bottom of the tube. how can I use material for antimicrobial test?
Try a different solvent & make sure to include a "solvent only" control.
Check the Merck Index for the solubility of the compound.
Please stop. Forcing the stuff into solution with some complicating solvent makes it totally irrelvant.
Consider how you would use a poorly-soluble material. Establishing efficacy on a surface maybe paper or cardboard with DMSO that you evaporate inoculate the surface with fungi and incubate in a humidity chanber
1st you can dissolve in DMSO after that add water. Before using you can use sonication. Every time try to prepare fresh stock.
you can use solvents like ethyl acetate and alcohols provided use only solvent as control to eliminate the solvent's activity.
It depends on which chemical class you are working with. Some water-insoluble substances dissolve well in DMSO, DMSO-Tween80 or alcohol.
Is your substance liquid or powder?
Try adding 1% DMSO and complete the volume with water. For example, if you're working with a final volume of 2.0 mL, you should add 20 uL of DMSO and complete the volume with water. If that doesn't work, try using 10% DMSO, which would be 200 uL of DMSO and 1800 uL of water. I work with liquid and water-soluble powder substances, and most of the time, DMSO works well. However, in some cases, I need to use another agent for dissolution. In cases where DMSO doesn't work, I use Tween 80, and it works well.
did you dissolve all material in Tween 80 or DMSO-Tween80 mixture you dissolve
Usually, when dealing with hydrophobic compounds, whether synthetic or standard antibiotics like amphotericin B, I weigh the required amount of powder and dissolve it solely in DMSO without adding water or other compounds. This results in my primary stock solution (mother stock). From this stock solution, I perform calculations to determine how much I need to pipette to prepare a substance at the desired test concentration
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