I want to get a K. lactis yeast condon optimized Cas9, which software or website can do that?
Hello,
I've used:
https://www.idtdna.com/CodonOpt
https://www.thermofisher.com/uk/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html#
Hi Dai, are you in China? You could order the optimized sequence to the Genescript company in Nanjing, and they will give the optimized plasmid back to you.
I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.
11 December 2018 701 0 View
I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote. Can anyone suggest me some papers? Thank you!
05 June 2017 4,554 0 View
How can the vectors be integrated into yeast genome as the rate of homologous recombination is low?
05 June 2016 4,860 5 View
I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...
03 April 2016 5,706 6 View
I want to express a protein in Kluyveromyces lactis, is the plasmid used for SC yeast OK?
03 April 2016 2,547 5 View
I want to add a tag to the 3' end of my target gene using Crispr-Cas9 system. What can I do if the appropriate sgRNA is far from the 3' end of the protein?
01 February 2016 2,751 2 View
I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...
02 March 2014 2,862 3 View
.
02 March 2014 1,937 0 View
Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?
01 January 1970 8,767 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View