How can I condon optimize a protein?

More Tianchun Dai's questions See All

How can I repress an endogenous gene expression?

I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.

11 December 2018 701 0 View

Which enzymes catalyze NMP, NDP and NTP transforamtion?

I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote. Can anyone suggest me some papers? Thank you!

05 June 2017 4,554 0 View

What is the mechanism of yeast integrative plasmid?

How can the vectors be integrated into yeast genome as the rate of homologous recombination is low?

05 June 2016 4,860 5 View

What is the promoter of Kanamycin in prokaryote plasmid?

I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...

03 April 2016 5,706 6 View

Can plasmid used for S.cerevisiae transformation be used for Kluyveromyces lactis?

I want to express a protein in Kluyveromyces lactis, is the plasmid used for SC yeast OK?

03 April 2016 2,547 5 View

How can I add a tag right bebind the 3' end of a gene?

I want to add a tag to the 3' end of my target gene using Crispr-Cas9 system. What can I do if the appropriate sgRNA is far from the 3' end of the protein?

01 February 2016 2,751 2 View

Can anyone help with vector construction with PGBKT7?

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02 March 2014 2,862 3 View

Can anyone suggest me a good cotton genome database?

02 March 2014 1,937 0 View

Are there any "master transcription factors" in yeast?

Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?

01 January 1970 8,767 1 View

Can I use a HisTRAP column for affinity chromatography?

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07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

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MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

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Problems with ion exchange chromatography?

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Why knockouts for same genes from two different gRNA are showing different result?

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How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

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How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

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Filippo Prischi

Hello,

I've used:

https://www.idtdna.com/CodonOpt

https://www.thermofisher.com/uk/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html#

Qinglin Yang

Hi Dai, are you in China? You could order the optimized sequence to the Genescript company in Nanjing, and they will give the optimized plasmid back to you.