How can I repress an endogenous gene expression?

I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.

11 December 2018 620 0 View

What is the mechanism of yeast integrative plasmid?

05 June 2016 4,813 5 View

How can I condon optimize a protein?

I want to get a K. lactis yeast condon optimized Cas9, which software or website can do that?

04 May 2016 6,034 2 View

What is the promoter of Kanamycin in prokaryote plasmid?

I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...

03 April 2016 5,452 6 View

Can plasmid used for S.cerevisiae transformation be used for Kluyveromyces lactis?

03 April 2016 2,497 5 View

How can I add a tag right bebind the 3' end of a gene?

01 February 2016 2,699 2 View

Can anyone suggest me a good cotton genome database?

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02 March 2014 1,888 0 View

Can anyone help with vector construction with PGBKT7?

I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...

02 March 2014 2,777 3 View

Are there any "master transcription factors" in yeast?

Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?

01 January 1970 8,719 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View