I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote.
Can anyone suggest me some papers? Thank you!
I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.
11 December 2018 701 0 View
How can the vectors be integrated into yeast genome as the rate of homologous recombination is low?
05 June 2016 4,860 5 View
I want to get a K. lactis yeast condon optimized Cas9, which software or website can do that?
04 May 2016 6,083 2 View
I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...
03 April 2016 5,706 6 View
I want to express a protein in Kluyveromyces lactis, is the plasmid used for SC yeast OK?
03 April 2016 2,547 5 View
I want to add a tag to the 3' end of my target gene using Crispr-Cas9 system. What can I do if the appropriate sgRNA is far from the 3' end of the protein?
01 February 2016 2,751 2 View
I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...
02 March 2014 2,862 3 View
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02 March 2014 1,937 0 View
Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?
01 January 1970 8,767 1 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....
31 July 2024 7,771 5 View
I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
31 July 2024 7,860 3 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View