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I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.
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I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote. Can anyone suggest me some papers? Thank you!
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How can the vectors be integrated into yeast genome as the rate of homologous recombination is low?
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I want to express a protein in Kluyveromyces lactis, is the plasmid used for SC yeast OK?
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I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...
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I want to add a tag to the 3' end of my target gene using Crispr-Cas9 system. What can I do if the appropriate sgRNA is far from the 3' end of the protein?
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I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...
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Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?
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