How can I repress an endogenous gene expression?

I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.

11 December 2018 620 0 View

Which enzymes catalyze NMP, NDP and NTP transforamtion?

I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote. Can anyone suggest me some papers? Thank you!

05 June 2017 4,440 0 View

What is the mechanism of yeast integrative plasmid?

05 June 2016 4,813 5 View

How can I condon optimize a protein?

I want to get a K. lactis yeast condon optimized Cas9, which software or website can do that?

04 May 2016 6,034 2 View

What is the promoter of Kanamycin in prokaryote plasmid?

I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...

03 April 2016 5,452 6 View

Can plasmid used for S.cerevisiae transformation be used for Kluyveromyces lactis?

03 April 2016 2,497 5 View

How can I add a tag right bebind the 3' end of a gene?

01 February 2016 2,699 2 View

Can anyone help with vector construction with PGBKT7?

I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...

02 March 2014 2,777 3 View

Are there any "master transcription factors" in yeast?

Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?

01 January 1970 8,719 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View