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I want to down-regulate the expression of a gene in yeast. Could you please suggest me some methods? Thank you.
11 December 2018 701 0 View
I want to know the mechanism of NMP, NDP and NTP transformation in eukaryote. Can anyone suggest me some papers? Thank you!
05 June 2017 4,554 0 View
How can the vectors be integrated into yeast genome as the rate of homologous recombination is low?
05 June 2016 4,860 5 View
I want to get a K. lactis yeast condon optimized Cas9, which software or website can do that?
04 May 2016 6,083 2 View
I can not find Kana promoter in the map, e.g., pet28, while AmpR promoter is always indicated. Then what is the Kana promoter sequence? And can E. coli containing a plasmid with KanMX(TEF promoter...
03 April 2016 5,706 6 View
I want to express a protein in Kluyveromyces lactis, is the plasmid used for SC yeast OK?
03 April 2016 2,547 5 View
I want to add a tag to the 3' end of my target gene using Crispr-Cas9 system. What can I do if the appropriate sgRNA is far from the 3' end of the protein?
01 February 2016 2,751 2 View
I want to insert the CDS of my gene into the MCS of PGBKT7, but I found that the vector contains two sites NdeI(CATATG) and NcoI (CCATGG), I would like to know whether the ATG in these two sites...
02 March 2014 2,862 3 View
Are there such TFs in yeast, like Myc, whose overexpression could affect multi-pathway protein expression?
01 January 1970 8,767 1 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
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24 July 2024 1,559 1 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
