I am supposed to determine the enzyme activity of endoglucanase at various time points.
I am supposed to use CMC as substrate and perform standard procedure that is used for DNSA assay , followed by taking spectrophotometric readings at 575 nm.
My question is how to co-relate the absorbance value that I get in DNSA method to that of enzyme activity (endoglucanase)
Please mention standard procedure if any one followed previously APART FROM THE FILTER PAPER APPROACH.
Kindly include any known standard manuals/ literature with detailed steps for performing the experiment.
Thank You.
You have to firstly plot a standard curve for reducing sugar by which you will get a graph factor.
Taking the above factor into consideration and your obtained reading for sample, you will have to express your results in terms of release of reducing sugars.
Also find the attached paper.
Thank you Sarang The article was a bible of cellulose assay information.
The following article have all the procedures for cellulase assay in details with calculations also. All The best.
-AKM
Hello Abhas thank you for the document attached, it would be helpful :) good day ...happy researching :)
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