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Questions related from Goutham Nageshwar
Case: I have performed inter laboratory comparison for 2 samples and obtained our lab and referral lab value in IU/ml. As per NABL guidelines (India) the acceptable range between the two values...
26 November 2020 4,250 1 View
Looking for midi and maxi plasmid isolation protocols from E coli (high copy number plasmid) cultures above 100ml (LB) Protocols tried so far: Sambrook's midi and maxi prep Alkaline lysis...
05 April 2018 1,583 3 View
I am working with development of viral diagnostic kits using Real Time PCR (Probe based) detection. As per standard procedures we do use plasmid carrying viral gene as control and to determine the...
30 November 2017 1,892 21 View
Are there any well established in-house methods for PCR product purification with out using any agarose gel elution methods/ commercial column based. Please suggest or share protocols and literatures
02 September 2017 5,994 6 View
What are the companies or government bodies in India who can provide international standards for Hepatitis B, C, and HIV which can be used for quantitiative measurement using Real -Time PCR .
24 June 2017 3,000 4 View
I wish to know sources in India from where I can get HBV/HCV samples for my research purpose. I work in a DSIR/NABH recognized laboratory and will be using them as reference standard for genetic...
06 March 2017 9,485 2 View
Please share protocols for faster and efficient lysis of RBC. Probable requirements: lysis buffer to sample (volume) ratio 1:4/1:2
20 January 2017 2,330 9 View
Product: 4-Methylumbelliferone. Company: SIGMA Cat no.M1381. Issue: According to the datasheet provided by sigma this compound gets dissolved in Methanol and Glacial Acetic acid. I did try using...
14 October 2016 5,883 3 View
I here attach the reference for bacillus transformation using paris method. It says MG1 and MG2 salts need aminoacid growth factor to 50 ug/ml and 10 ug/ml. I do know yeast extract is a source of...
19 July 2016 9,228 2 View
I am working with Bacillus subtilis RIk1285 (derivative of 168 W.T from TaKaRa), the maximum O.D I get when cultured in L.B broth (Flask work) is 4 O.D and in 7.5 Litre bench top fermentor...
10 June 2016 899 3 View
I have been trying to dilute Cisplatin in 0.9% PBS, but when ever I dilute it instead of completely getting diluted the tube resides some yellow colored minute powdered particles, they get...
30 July 2015 2,997 3 View
I am supposed to determine the enzyme activity of endoglucanase at various time points. I am supposed to use CMC as substrate and perform standard procedure that is used for DNSA assay , followed...
02 July 2015 6,545 4 View
Kindly some one let me know the amplicon size of this TA vector(control circular plasmid with out insert provided with kit ) from LIFE TECHNOLOGIES when amplified with M13 F` and R` primer . I...
11 June 2015 5,478 3 View
Kindly let me know if i can autoclave trypan blue solution as I wish to add it into Agar plates to see clear zones for cellulase activity. I also have to add CMC to the agar plates, so is 1% CMC...
16 March 2015 7,177 3 View
Hello I am trying to amplify a stretch of Double stranded DNA (of 1.4 Kb) using A forward primer of 112 base pairs and reverse of 21 base pairs. The Tm value of the 112 basepairs long Forward is...
10 January 2015 9,992 26 View
Any literature evidence suggesting specific genes responsible/known to trigger the differentiation of a cancer stem cell (in any cancer) back to cancer cell kindly provide the reference and...
08 October 2014 8,625 2 View
