Abhas Kumar Maharana

12 Questions 57 Answers 0 Followers

Questions related from Abhas Kumar Maharana

What should be the appropriate composition of dinitro-salicylic acid reagent for reducing sugar determination?

There are many literature that enlightened regarding this but I have some doubts as follows: 1. Introduction of phenol to the reagent, can intensify the color. What it means actually it will give...

22 November 2015 8,965 8 View

What is the impact of music on growth of microorganisms? Is there any relationship?

Music and sounds can strongly affect our emotions and mood. Even if by listening some songs one's evilness also cured. Are microbes respond the same as we or not ? There are very few reports on...

29 August 2015 4,512 17 View

What are the major reasons for getting extremotolerants while working on extremophiles?

Generally what happened, we are giving extreme conditions to them for initial isolation but later we have only "tolerants" not the "true philes". Why?? Thank you in advance.

12 August 2015 8,380 8 View

Why the media (Horikoshi media for alkalophiles) turns black after autoclave?

The media composition: Glucose (10g), yeast extract (5g), Peptone (5g), K2HPO4 (1g), MgSO4 (0.2g), Na2CO3 (10g), dw 1L and agar 2%. The pH of the medium was automatically adjusted to 10pH. The...

22 January 2015 7,489 13 View

What is the reason behind this color change (blue) of CMC-congo red agar medium during cellulase assay?

I used CMC congo red agar for the screening of cellulase using Aspergillus terreus. From the plate it is found that there is a clearance of CMC and with that there is a blue color development (see...

20 January 2015 1,248 7 View

How can I isolate the obligate alkalophiles from sea water and sediments?

It is always happened that while working on any extremophiles we got extremotolerants first. Here in this case I want to isolate obligate alkalophiles from sea sediments and water. I went through...

09 January 2015 1,804 4 View

In the Protease assay, which substrate (Azocasein or casein) and method should be taken?

I followed some literature concerning the protease assay but I am a little bit confused about what substrate should be taken for the assay. Yes, casein is cheaper than azocasein, but is there any...

27 December 2014 2,757 12 View

Where to submit industrially important organisms? What is the procedure of culture submission?

My isolated micro. are good producers of cold active enzymes like (lipase and endoglucanase). Where to submit those cultures? I heard about MTCC. Is there any other options for this? Which is the...

04 November 2014 1,415 2 View

Why is there a reduction in the production of lipase when there is an increase in oil concentration?

While optimizing the lipase production, oil concentrations (tributyrin oil) from 5-25% v/v were taken, and it was observed that enzyme activity was decreased with an increase in oil...

25 October 2014 4,167 6 View

Why does EDTA act as an inhibitor for some enzymes but not others? What is the mechanism of action of EDTA on enzyme activity?

While working on cold active lipase and endoglucanase I found that both enzymes can tolerate EDTA for 1 hr incubation (stability study of the enzyme). But much of the literature said EDTA acts as...

25 October 2014 6,350 10 View

What is the relationship between enzyme hydrolytic zone and growth of filamentous fungi in relation to temperature?

While working on filamentous fungi for the production of cellulase enzyme, more hydrolytic zone was seen in case of CMC plate incubating at 15 degree cel. than 35 degree cel. But the growth of...

23 September 2014 6,765 5 View

Why is Serratia marcescens red at 25 degree but white at 37 degree Celsius?

Serratia marcescens can tolerate and grow at both temperature mentioned above but why there is a variation in pigment while temperature varies? What is the mechanism behind this? Many...

21 September 2014 6,350 6 View