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Questions related from Abhas Kumar Maharana
There are many literature that enlightened regarding this but I have some doubts as follows: 1. Introduction of phenol to the reagent, can intensify the color. What it means actually it will give...
22 November 2015 9,210 8 View
Music and sounds can strongly affect our emotions and mood. Even if by listening some songs one's evilness also cured. Are microbes respond the same as we or not ? There are very few reports on...
29 August 2015 4,638 17 View
Generally what happened, we are giving extreme conditions to them for initial isolation but later we have only "tolerants" not the "true philes". Why?? Thank you in advance.
12 August 2015 8,461 8 View
Simple answer is to grow them on agar medium with respect to different temperature from 0-100°C. If the growth range is 0-20°C then psychrophiles, if 0-40°C then psychrotolerant and if 20-45°C...
23 February 2015 5,402 11 View
The media composition: Glucose (10g), yeast extract (5g), Peptone (5g), K2HPO4 (1g), MgSO4 (0.2g), Na2CO3 (10g), dw 1L and agar 2%. The pH of the medium was automatically adjusted to 10pH. The...
22 January 2015 7,552 13 View
I used CMC congo red agar for the screening of cellulase using Aspergillus terreus. From the plate it is found that there is a clearance of CMC and with that there is a blue color development (see...
20 January 2015 1,317 7 View
It is always happened that while working on any extremophiles we got extremotolerants first. Here in this case I want to isolate obligate alkalophiles from sea sediments and water. I went through...
09 January 2015 1,876 4 View
I followed some literature concerning the protease assay but I am a little bit confused about what substrate should be taken for the assay. Yes, casein is cheaper than azocasein, but is there any...
27 December 2014 2,860 12 View
My isolated micro. are good producers of cold active enzymes like (lipase and endoglucanase). Where to submit those cultures? I heard about MTCC. Is there any other options for this? Which is the...
04 November 2014 1,465 2 View
While optimizing the lipase production, oil concentrations (tributyrin oil) from 5-25% v/v were taken, and it was observed that enzyme activity was decreased with an increase in oil...
25 October 2014 4,237 6 View
While working on cold active lipase and endoglucanase I found that both enzymes can tolerate EDTA for 1 hr incubation (stability study of the enzyme). But much of the literature said EDTA acts as...
25 October 2014 6,435 10 View
While working on filamentous fungi for the production of cellulase enzyme, more hydrolytic zone was seen in case of CMC plate incubating at 15 degree cel. than 35 degree cel. But the growth of...
23 September 2014 6,867 5 View
Serratia marcescens can tolerate and grow at both temperature mentioned above but why there is a variation in pigment while temperature varies? What is the mechanism behind this? Many...
21 September 2014 6,436 6 View
