How to evaluate inductance of a solenoid in accordance with the air gap?

Hello All, I am currently modeling a solenoid on Matlab. I have evaluated the inductance of the solenoid based on its geometrical configuration using the formula, L = mu0*n^2*V where, mu0 =...

16 February 2021 5,613 5 View

How to define acceptable log10 range for real time pcr quantitative assay?

Case: I have performed inter laboratory comparison for 2 samples and obtained our lab and referral lab value in IU/ml. As per NABL guidelines (India) the acceptable range between the two values...

25 November 2020 4,112 1 View

How to run the MD simulation for a protein-ligand system in NAMD?

Hi I am trying to run the MD simulation for protein-ligand system in NAMD. But I am having troubles running it. It says the ligand is not parametrised when generating psf file. I understood the...

28 August 2019 9,774 2 View

Validation of docking result?

hello. i have finished running the ensemble docking with ligand library. what are the next step of computational methods or other methods available to prove the top scored molecules likely to be...

08 March 2019 3,175 7 View

Which is the best inhouse protocol for midi and maxi prep plasmid DNA isolation?

Looking for midi and maxi plasmid isolation protocols from E coli (high copy number plasmid) cultures above 100ml (LB) Protocols tried so far: Sambrook's midi and maxi prep Alkaline lysis...

04 April 2018 1,377 3 View

What can improve copy number detection sensitivity of real time pcr FAM-BHQ1 probe based?

I am working with development of viral diagnostic kits using Real Time PCR (Probe based) detection. As per standard procedures we do use plasmid carrying viral gene as control and to determine the...

29 November 2017 1,758 21 View

Would like to know where I can buy the standards for HBV,HCV, HIV to perform Real-Time PCR based diagnosis?

What are the companies or government bodies in India who can provide international standards for Hepatitis B, C, and HIV which can be used for quantitiative measurement using Real -Time PCR .

23 June 2017 2,855 4 View

How to calculate acoustic emission signals to stress generation while machining

I want to calculate stress generation with respect to load acting on the member while machining with abrasive waterjet machine by using AE signals. Can anyone help?Thanks

27 February 2017 5,678 3 View

Efficient and fast RBC lysis protocol.? (NOT Commercial kit method)?

Please share protocols for faster and efficient lysis of RBC. Probable requirements: lysis buffer to sample (volume) ratio 1:4/1:2

19 January 2017 2,158 9 View

Name few best fermentation medium compositions to attain high cell density in Bacillus subtilis strains.?

I am working with Bacillus subtilis RIk1285 (derivative of 168 W.T from TaKaRa), the maximum O.D I get when cultured in L.B broth (Flask work) is 4 O.D and in 7.5 Litre bench top fermentor...

09 June 2016 768 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View