Why pet plasmids can't replicate in pseudomonas?

26 June 2024 1 7K Report

Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and 21 can't replicate in to pseudomonas?

Thanks for regards

Michael J. Benedik

They don't replicate in Pseudomonas because the origin of replication does not function. This is because a promoter that makes the primer for replication is not recognized in Pseudomonas.

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