How can get 3D structure of my desire protein sequence in PDB format??

03 March 2016 1 2K Report

I need PDB format of my desire proteins. I have their sequence. how can I get me question solved?

You may use Uniprot data base to find similarity of your sequence. Also, might use SWISS-MODEL Workspace for the generation of 3D structure in PDB format.

Badges
Science topic

More Ali Akbar Jamali's questions See All

What are the microRNA-drug interaction databases?

I am looking for databases that contain microRNA-drug interactions. Any suggestions or recommendations?

09 October 2019 5,374 4 View

MicroRNAs in Breast cancer?

I need to get the list of microRNAs involving in breast cancer. is there any review paper, database, online tool, etc.?

06 July 2018 4,772 0 View

Software for Drug and miRNA docking simulation?

I need to study the interaction between some drugs and miRNAs. I was wondering if someone can tell me how can I perform this simulation? Is there any software? or how can I perform this simulation...

05 June 2018 5,895 7 View

How to calculate drugs/compounds similarity?

I need to construct n*n similarity matrix of n drugs/compounds. I need an online or offline tool to construct this matrix. I have the SMILE of drugs/compounds. any suggestion or recommendation?

11 December 2017 793 2 View

Where & How can I get full 3'-UTR sequences for Bos taurus?

I need full 3'-UTRs for Bos taurus. who knows where I can get this fasta file? please share any link if is possible.

10 November 2014 5,230 0 View

Where can I get validated negative interaction?

I'm in need of experimentally validated microRNA negative interaction. I got validated positive interactions from mirtarbase. But I don't know where to get negative ones. Please share if you have...

03 April 2014 749 1 View

What is the difference between microRNAs with star and without star?

For example what's the difference between these 4 microRNAs? hsa-miR-101* hsa-miR-101 hsa-miR-101-3p hsa-miR-101-5p Which pairs (if) are the same? Which pairs are the same in sequence?

04 May 2013 6,939 4 View

What's difference between "hsa-let-7a-5p" & "hsa-let-7a-3p"? What do 5p & 3p refer to?

04 May 2013 10,086 9 View

How can I ensure that one microRNA doesn't regulate a specific gene or mRNA?

I need some negative data for running my model. These negative data consists of microRNAs and genes that don't interact with each other. How I get this negative data?

04 May 2013 1,830 4 View

how can I convert Fastq to SAM file?

dears: I'm working on chip-seq data. I've downloaded sra format data. to analyze I have to convert them to fastq format and then to SAM file. I made a fastq file but I cant convert it to SAM file?...

11 December 2012 4,331 14 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View