I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate.
Can anyone share me article/s relating apoptosis and micronuclei?
How exactly is cell undergo apoptosis and formation of micronuclei and its mechanism?
Does it relating to toxicity of compound against cell?
What is the size of micronucleus?
Thank you,
7-AAD (7-Aminoactinomycin D) is the best in DNA analysis for flow cytometry because it is DNA specific binding.
Acridine orange is less specific because it binds also to lysosomes.
Ethidium bromide is lesser specific because it binds to DNA and RNA.
Here attached you will find all your questions about cell death pathways.
Best regards
Robert
Acridine orange combined with Pserine assay gives you an idea about how may cells die of apoptosis at a low cost...please consult Jamison Jm et al
If acridine only marks the nucleus it is necrosis or autoschizis, if it marks nucleus and phosphatidylserine altogether and then the nucleus appears yellow through the green if green used, then apoptosis is there. This can be used for flow cytometry.
Using acridine orange, you can analyze micronuclei (green fluorescence) and apoptotic cells (cytoplasm emitted orange/yellow fluorescence) with fragmented nucleus (green fluorescence)
acridine orange fluorescence : [DNA - green fluorescence; RNA - orange/yellow fluorescence)
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