How to analyse GC MS spetra?

Kindly help to interpret GC-MS of plant extract? how to relate a particular compound from the spectra. What is important point to consider? Thank you all

09 October 2018 7,321 5 View

What gene will be suitable for molecular Plant identification?

Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?

08 September 2018 5,134 0 View

Can anyone suggest AntiCancer research approach?

Dear Experts, I would to carry out a research on Plant source medicinal or ethno-pharmaceutical in vitro and in vivo. I would to focus on Biochemical-Physiological profiling and molecular...

06 July 2018 7,448 0 View

Can cancer cells from animal origin develop in human?

Can cancer cells from animal origin growth, develop and proliferate in human? What if not, what exactly is the reason? is it immune system incompatible alone for not proliferation? Thanks

07 August 2016 6,356 1 View

Is it sufficient anova way statistical analysis?

Dear researchers, I need to perform biochemical experiment data to analysis statistical significance. I have more two groups for all experiments. But I am not sure what statistical parameter will...

03 April 2016 1,818 3 View

Can anyone share me some information on western blot?

Respected Researchers, I would like to know what is the principle behind the Western blotting? How will be the expression of interest protein if treated cancer cell with drugs? I read many...

03 April 2016 3,759 2 View

Kindly help me with western blotting?

Researchers, can anyone suggest me western blotting technique? I need to perform western blotting but i have no practical experienced yet. How to develop the band or expression of protein on...

02 March 2016 3,458 6 View

What stastistical parameters will be appropriate for biochemical assays?

02 March 2016 1,113 6 View

How acurate is Acridine orange/ ethidium bromide detection of apotosis and micronuclei?

I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...

01 February 2016 6,592 5 View

Experts, can anyone suggest me western blotting technique?

I need to perform western blotting but i have no practical experienced yet. Please kindly suggest simple and accurate procedure for Western Blot. And what dye/stain will is reversible for protein...

01 February 2016 10,037 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View