Hi, I'm doing the alkaline comet assay but I usually find halos around the cells and not comets. Does anyone know why this happens?

My lysis Buffer contains 5M Nacl, 0.5M EDTA, 1M tris ph 10, 10 MNaOH, Then 1% triton and 10% DMSO is added on the day of the experiment. Electrophoresis Buffer contains 0.5M EDTA, 10M NaOH and 1%DMSO

25 September 2019 7,131 1 View