Samrat Das

3 Questions 40 Answers 0 Followers

Questions related from Samrat Das

ABCA1 Western Blot ?

Hello everyone, I am trying to visualise ABCA1 protein (which is approximately 220-240 KD) by western blot from my experimental in-vitro cell samples. To extract proteins, I use either: NucleoSpin...

17 October 2024 9,142 4 View

What is the effective minimum dose of PHA to activate cell cycle progression in human lymphocytes ?

In many articles i found that approximately 40µg/ml (dilution) of PHA has been used for stimulation of lymphocytes. My question is whether this dose is appropriate for lymphocyte activation...

10 April 2017 9,198 2 View

Very high % of G2-M phase cells and very less % of "g0/gi" and "s" phase cells in some of the test sample(s) as compared to control group..reasons?

In some of my experimental test group samples i am observing high G2-M phase cells. Does that indicate that most of the cells are able to go through the g0/gi and s phase successfully and there is...

25 June 2016 2,021 4 View