How to increase DPI in my image?

I'm submitting manuscript to a journal that requires 1200 dpi in my images. I have changed the dpi from 96 but when I upload the image with 1200 dpi, the image looks super blurry when I review my...

16 February 2021 2,803 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

17 January 2021 5,516 3 View

What are some examples of practice based methods and techniques that can be used to study social dance styles?

I am currently developing a project where the primary mode of inquiry is rooted in practice based methods and techniques. The project is focused on a social dance form. I have read several...

23 March 2020 793 5 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

How does being a teacher influence creative processes in creating choreography?

I would be interested in resources that pertain to how identity or the different roles we possess in dance making (dancer, teacher, spectator, mentor, etc.) influence creative processes in...

06 December 2019 8,064 2 View

Can anyone recommend any articles with clear ubiquitin staining in cells?

My ubiquitin staining is fairly variable and I've been looking for published articles containing such stainings so I can see how it normally looks. I've had some trouble locating any good sources...

14 September 2019 8,904 1 View

Western blots on tissue which have been stored in RNAlater?

I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen). I was wondering if anyone had any experience with running western blots using tissue that has...

28 August 2019 7,930 3 View

Ecological Validity: How can experiments that take place in a social setting measure validity and reliability?

Thinking in terms of a social setting such as a dance, a concert, a meal, if an experiment were to be designed in such a way, how can the method be validated? Similarly, what role would...

26 August 2019 3,415 3 View

Does the volume of a song influence our enjoyment of it?

If a song is played louder does it increase or decrease our enjoyment towards a song? Do we have to already enjoy or dislike a song in order to notice the influence of volume? Any possible...

22 August 2019 2,089 11 View

What are the best conditions for transferring a >200kDa and ~40kDa protein?

I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other...

22 August 2019 1,081 7 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View