Irfan Khan

3 Questions 26 Answers 0 Followers

Questions related from Irfan Khan

Can anybody explain bad results from PCR for microRNA?

I am doing PCR for microRNA, I isolated total RNA, then using stem loop primer, I make cDNA, and now doing PCR with bioRad cyber green kit. Some of my microRNA sample have very good melting curve...

05 May 2014 4,201 5 View

How to design primer for piRNA?

I am planning to detect expression of piRNAs in human stem cells. Can anybody please tell me how to design primers for piRNAs?

04 April 2014 4,737 1 View

What kinds of growth supplement can be used for Human MSCs growth for transplantation?

We plan to conduct one study for Diabetic wounds regeneration by adipose derived Mesenchymal Stem Cells from a patient's own adipose tissue. For this purpose we plan to isolate and grow MSCs from...

18 February 2014 5,223 15 View