I'm testing if particular plant and mushroom extracts inhibit the BACE-1 enzyme from cleaving APP, and I'm not sure how to make the extracts.

I would ideally like to test polysaccharides.

All of my plants and mushroom samples are polar, so I think I should use some type of alcohol, but I'm unsure of which one and which process.

How do you make extracts of mushrooms compared to plant samples (like roots and leaves)

Thank you!