HELP!
Our lab has recently discovered these dancing blobs in our TC cultures - across cell lines, primary cells and organoids. So far they are not behaving like any bacteria/fungi/yeast we have ever come across (not responding to antibiotics/antifungals and no turbid media). They seem to be amorphous and both extra/intracellular..
Someone has suggested they may be protozoa? If anyone has seen something similar or is an expert microbiologist please help us identify them!!
(Picture included for attention but really need to watch the videos to distinguish from cells/debri)
Hi Olivia Craig ,
If the dots-like particles in your cell cultures are moving then they are Achromobacter species, you can use antibiotics as per your lab manual. I have used piperacillin at 0.5- 10 microgram/ml concentration. replenish the media every day till the complete removal of these particles. Try it. It will work.
Thank You
Sameer
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