I messed up. Will whole cichlid brains left in 4% PFA for a week be useable for IHC?

26 December 2023 0 4K Report

Hi,

I am using IHC to stain for DNA damage and a lipid peroxidation byproduct (4HNE) in small fish brains

I accidentally left my (whole) brains in 4% PFA at 4C for A WEEK after placing them in there immediately after dissection. I just placed them in 30% sucrose and put them back at 4C to move to embedding matrix tomorrow for cryosectioning at a later date.

Will this be okay? What should I do?

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