No title

26 December 2020 1,951 3 View

What solvent mixture/ratio should I use for preparing liposomes by REV method?

I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.

24 February 2020 2,360 5 View

How to analyze comparatively the data from several similar surveys?

We have conducted three representative surveys with the same questioner on high school students from three different cities. The cities have different characteristics from ethnic, economic and...

19 August 2019 2,920 6 View

What is the best RNAse inhibitor?

Hello Everyone! I need to send some RNA samples to sequencing, and I want to know which RNAse inhibitor do you use to ensure that your samples don't degrade during shipping and how do you use it...

09 October 2018 4,888 4 View

A good p-IR antibody for WB?

Hello! I work with mouse islets and I want to detect phospho-Insulin Receptor by western blot but all the antibodies I have been testing are horrible... even with amounts of protein around...

25 January 2018 9,101 2 View

Do anyone knows where to buy a frozen culture of TPC1-papillary thyroid carcinoma cells?

It seems that people got them as gifts from collaborators, but not in a comercial distributor...all info is welcome :)

17 January 2018 5,345 2 View

Do anyone knows where to buy a frozen culture of TPC1-papillary thyroid carcinoma cells?

It seems that people got them as gifts from collaborators, but not in a comercial distributor...all info is welcome :)

31 December 2017 2,680 2 View

Where could I find raw public databases for TOL-DX (Tower of London - Drexel) tests?

For my research, I would like to have access to real data from Tower of London tests. I haven't been able to locate database sources such that we can try our AI algorithms on them. Thanks in advance.

10 November 2017 4,846 1 View

Are the high volume fly ash concretes better or at least as good as common concrete?

05 September 2017 8,326 13 View

Has anyone experienced on siRNAs transfection in B-CPAP cell line?

I am gonna start working with this thyroid cell line and I was wondering if anybody knows if they are "easy" for tansfection,growth and so on. In addition, do you used siRNAs with them? Thanks to...

08 September 2016 8,939 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View