A good p-IR antibody for WB?

Hello! I work with mouse islets and I want to detect phospho-Insulin Receptor by western blot but all the antibodies I have been testing are horrible... even with amounts of protein around...

25 January 2018 9,101 2 View

Do anyone knows where to buy a frozen culture of TPC1-papillary thyroid carcinoma cells?

It seems that people got them as gifts from collaborators, but not in a comercial distributor...all info is welcome :)

17 January 2018 5,345 2 View

Has anyone experienced on siRNAs transfection in B-CPAP cell line?

I am gonna start working with this thyroid cell line and I was wondering if anybody knows if they are "easy" for tansfection,growth and so on. In addition, do you used siRNAs with them? Thanks to...

21 September 2016 2,549 1 View

What is the best programming language for an open-source computer vision project? Which one would you prefer as potential developer?

We have recently developed a tracking system capable of following several individuals keeping their correct identities in spite of crossings or overlaps (www.idtracker.es). It will be published in...

13 May 2014 9,685 72 View

What is the best programming language for an open-source computer vision project? Which one would you prefer as potential developer?

We have recently developed a tracking system capable of following several individuals keeping their correct identities in spite of crossings or overlaps (www.idtracker.es). It will be published in...

04 May 2014 6,634 75 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View