Hi, You could try the following kit:

http://www.activemotif.com/catalog/25/nuclear-complex-co-ip-kit

However, be aware than although the manual does not implicitly tell you, you should optimise the amount of detergent you add in the hypotonic step. The kits is loosely based on the paper attached, and you need to use less detergent than they state otherwise you will get your nuclei to lyse and get a viscous mess!

The nuclear/cytolplasmic prep from the paper also works very well and we used it in our recent paper (link to paper also attached).

Good luck. 

Article FAT1 cadherin acts upstream of Hippo signalling through TAZ ...

I always use a homemade recipe to perform co-IP. It never disappoints me. You may find the protocol here if you'd like to have a try.

https://www.protocols.io/view/Immunoprecipitation-dkm4u5

https://www.protocols.io/view/Immunoprecipitation-Lysis-Buffer-dkr4v5?guidelines

Thanks a lot! I gave the Biovision kit a shot since it was what I had in the lab, (I lysed the nuclear fraction lysed with IP buffer)... I am waiting on the results, if they are negative I will check the recipes and kit suggested! Much appreciated.

FYI the co-IP worked beautifully with the biovision kit and IP lysis buffer.

Hi Reinaldo,

Can I ask what you were immunoprecipitating? I need to go down this track as well, with two transcription factors so it would be good to get your ideas. 

Do I understand you correctly from your comments that you did use the biovision kit first to isolate nuclei, then you used the Pierce reagents?

Thanks,

Yeah, that is correct. I used the Biovision kit but I did not add DTT to the buffers. I used around 10 million cells and scaled appropriately, based on the protocol. I was however very delicate while breaking the cytoplasmic fraction. I also washed twice with the suspension buffer from the kit to remove all cytoplasmic remnants before re-suspending the nuclear fraction with IP lysis buffer.

after nuclear re-suspension I vortexed on the lowest setting 3 times and put the samples on rotator at 4C for about 45 minutes. centrifuged, moved supernatant to new tube and started the co-IP as usual.

I was puling down HA-Nek2 and I used dynabeads covalently attached with the HA IgG.

Hope this helps!

Thanks Reinaldo!