Strongyloides or "Hookworm" (unusual presentation) larvated ova from canine faeces?

03 December 2023 0 7K Report

A single egg was isolated from a young dog faecal sample. The dog is showing GI signs.

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Science method

More Ross Ashley Machin's questions See All

How to correct an article ttle error?

The following article of which I am a co-author has a word missing from the title in ResearchGate: "Authigenic formation in suboxic marine sediments" should be "Authigenic magnetite formation in...

28 January 2024 4,296 0 View

How can I remove a published paper from Research Gate and replace it with an updated paper?

see question

16 January 2024 1,316 3 View

Best way to preserve FACS enriched cells for downstream single nucleus RNA & ATAC seq?

I am working with a cell population that needs to be enriched by cell sorting from fresh tissue. I am wondering what the best way to preserve the cells, after sorting, for downstream single...

30 October 2023 9,156 0 View

Why is my gel loading not consistent?

I loaded the 1.5% agarose gel as standard. I loaded 6uL from PCR and 1uL loading dye. Why is the solution not sinking to the bottom of the wells?

23 October 2023 3,884 2 View

My ligation troubleshooting gel has very blurry bands, what does this mean?

The first lane is 1kb ladder, the second lane is the ligation reaction, the third lane is plasmid only control. Previously the same PCR product gave clean bands but now it isn't and I don't know why.

02 October 2023 2,353 1 View

How do I decide the digestion time of PCR products?

My supervisor suggested that the bp of PCR products is related to the digestion time. I cannot find anything on the internet to solve my problem. I have a PCR product 500bp. My supervisor implied...

27 September 2023 2,887 3 View

Why are there no bands on the gel for my ligation?

I have run PCR and digestion (Not1-hf & Nde1) that gave good bands. The plasmid was PET-28-make123 and the PCR product was franken-flag-dsred. Then I performed ligation and ran a gel with...

21 September 2023 8,627 3 View

Protocols for purification of of LC-MS grade solvents?

We have recently been finding that some of our LC-MS grade solvents have impurities (several brands) - notably acetonitrile and methanol. To avoid future problems, we would like to do an in-lab...

08 August 2023 883 4 View

What number of intronic basepairs adjacent an exon would be important to look at for detecting issues with splicing/etc?

We are running a project where we are Sanger sequencing gDNA for a specific gene (DPYD), and doing sequencing reactions for each of the exons. The reads we get include intronic bp on each side of...

17 July 2023 4,885 2 View

How can I edit incorrectly entered publication details on ResearchGate?

How can I edit incorrectly entered publication details on ResearchGate? After a lay-off from keeping my publications entries up to date, I know I have made errors in recent listings. I would like...

14 July 2023 6,716 1 View

What is Ala Vestibuloplasty dog technique?

procedure in which both the axial portion of the alar wing and all of the alar fold are removed by amputation. The procedure results in a very cosmetic appearance when done properly.

16 May 2024 9,827 0 View

What information to focus on in cohort study about intra-articular repair of cranial cruciate ligament?

Dear ResearchGate community, My coauthors and I have been running a cohort study on intra-articular repair of cranial cruciate ligament in dogs. This clinical study includes 30 client-owned dogs...

01 May 2024 699 0 View

What is your favourite pet ?

Dog, cat

20 April 2024 4,685 2 View

What name is your dog?

Gon

25 March 2024 8,329 1 View

Do dog owners really know their pets?

Hello, my name is Manahil, and I am a Foundation Veterinary Nursing student, studying at Plumpton College. For my final year research project, I am conducting a study into how well dog owners know...

06 March 2024 7,130 5 View

How long can live tissue survive (in refrigeration 4℃) once removed from body?

I'm currently working with animal tissue, more specifically visceral fat tissues from dogs. Our labs are not equipped to function 24/7 at the moment so I have to make sure the sample collection...

25 February 2024 2,834 4 View

What native antibody is found in Het3 mice?

I am trying to determine if Het3 mice produce antibody to a compound. This compound should be non-immunogenic. They have not been exposed to anything else. Apart from injecting mice with an...

18 February 2024 5,271 0 View

Is anybody has had an issue generating membrane-bound OVA expressing cells in murine model?

Hello dear colleagues, I am trying to generate OVA expressing cell lines at the membrane surface in CT26 or B16F10 cells and my first assay are completely unsuccessful (only 4 positive clones for...

08 February 2024 8,130 0 View

What is Ala Vestibuloplasty dog technique?

A procedure in which both the axial portion of the alar wing and all of the alar fold are removed by amputation. The procedure results in a very cosmetic appearance when done properly.

04 February 2024 2,372 0 View

Please elaborate ACT test in dogs ?

answer this.

02 January 2024 3,327 1 View