Has anyone attempted to adapt UMNSAH/DF-1 (Chicken Fibroblast cells) into suspension cells?

06 May 2024 1 3K Report

Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting from 50:50 adaption stage. Can anyone suggest any growth factor to enhance the growth or any other media form adaption to suspension cells.

B.. A..

Does CEF Consider as feeder cell ?

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