Ashwin Naidu

6 Questions 4 Answers 0 Followers

Questions related from Ashwin Naidu

Has anyone attempted to adapt UMNSAH/DF-1 (Chicken Fibroblast cells) into suspension cells?

Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...

06 May 2024 3,275 1 View

Seeking Advice: Stagnant Cell Growth in Fed Batch Process Despite Daily Feeding - What Could Be the Issue?

I'm encountering a persistent problem with cell count stagnation in my fed- batch process with CHO S cell line. Initially, after seeding on day 5-6, the cell count reaches 6-8 million cells/ml,...

21 March 2024 8,594 6 View

Need suggestions on bioreactor parameter optimization and PID settings?

Hi, I have established a bioreactor parameters mammalian cell process with the following parameters: Setpoint Deadband PID settings 1) pH-...

15 December 2023 7,625 1 View

Effect of (Kolliphor) Poloxamer 188 on CHO cells?

In my experiment, I utilized a combination of Poloxamer 188 (Kolliphor) and Gibco Anticlumping agent in Dynamis media at a concentration of 0.1% per liter for CHO S cell culture. Surprisingly, I...

08 June 2023 7,476 1 View

Why is cell clumping observed in the CHO-S cell line despite the presence of an anticlumping agent?

What is the reason for cell clumping in the CHO-S cell line despite the presence of an anticlumping agent (1%/L) in Dynamis medium, when the cell density reaches above 3x10^6 cells/mL? Is the...

04 May 2023 7,471 3 View

Can we make an in house cell culture media for CHO - S cell line?

I'm just curios if we can make our own cell culture media for the CHO-S cell line. I went through some of the sites for custom make a cell culture media for mammalian cells, and only Cytiva could...

23 November 2022 5,793 3 View