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Questions related from Belinda Lago Vallejo
I'm encountering difficulty in achieving a purified extract of erythrocyte membrane proteins using a lysis procedure with 0.1% Triton, followed by multiple centrifugation steps at 12000 g and...
04 April 2024 7,681 2 View
I would like to measure the oxygen consumption rate directly from tissue in the seahorse XFe96 analyzer with the Seahorse XFe96 Spheroid FluxPak (Part Number:102905-100) but i need a specific glue...
03 April 2024 8,698 1 View
On the protocol, in the plate map, accidentally I named some wells with cells as background. I have the correct background wells applied, my problem is I added three more backgrounds in wells with...
28 March 2023 4,896 2 View
Im doing ELISAs with a number of samples and then I have my results as ODs and AUs. When i repeat the exactly same ELISA with the same samples, i see the ODs change. My samples come from patient...
21 October 2022 2,449 2 View
Is it necessary for the centrifuge to be 4ºC during the process? Do I have to add PBS or other kind of buffer before adding my protein sample? If the protein I want to concentrate is 60kDa for how...
06 September 2022 2,062 0 View
