Ho necessita di fare il western blot su campioni di liquido sinioviale, ma non so come estrarre le proteine, qualcuno può aiutarmi con il protocollo di estrazione?
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View
Do you think the use of virtual reality with dyslexia students is good?
23 June 2024 8,437 2 View
I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.
22 June 2024 4,859 8 View
I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...
21 June 2024 3,305 6 View
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19 June 2024 2,125 3 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...
16 June 2024 1,116 3 View
Please note, that this is my first manuscript under the name Mária Čápová. Then I have been married, and my name change to Plachá, but I would like to have this article in my profile. Ontogenetic...
16 June 2024 7,178 0 View
I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...
16 June 2024 1,506 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I want to know more about Mn deposits in west of Afghanistan.
07 August 2024 3,427 1 View
I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me
30 July 2024 3,743 0 View
I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?
18 July 2024 2,696 1 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View