In one paper that I´m currently reading, I found that these author creating reporter mice to to trace cells that involve in tissue repair in kidney.
In this study, they cross Six2-GFP-Cre mice with those who carry reporter genes as following (Picture below).
My question is: why do they use GFP-Cre here? Why not just using Six2-Cre? Would this GFP would interfere with other fluorescent signals if you use other fluorescent reporter genes?
I´m not familiar with generating reporter mice, so I will highly appreciate kind help/explanation from you, who are more experienced than me.
Thank you very much in advance.
Mai
(The paper I referred to: . Humphreys et al. (2008). Intrinsic Epithelial Cells Repair the Kidney after Injury. Cell Stem Cell 2, 284–291)
I inow this paper well. I think there is no need to use Six2 GFP-Cre, they could have used Six2-Cre for the lineage labeling. I think this was the line available at the time. Also it has the added benefit that you can see where Six2 cre is expressed and where it is not. In any event GFP will not interfere with the detection of dsRED or lacZ. Most modern microscopes with proper filters can easily separate out the various fluorescent signals.
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