How reliable are predicted sequences?

If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?

11 December 2017 5,640 0 View

Are humoral immune indexes such as lysozyme and antiproteases measured in terms of Opitcal density or Optical depth?

In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...

09 October 2016 8,552 2 View

Antiprotease activity, when a measure is negative, what could be the source of the problem?

My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...

08 September 2016 9,796 5 View

How long can sera samples from fish be stored and at what temperature?

I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.

08 September 2016 600 5 View

Why is the acute toxicity of many drugs measued using LD50?

07 August 2016 9,135 0 View

Which is the best time to take the weight of fish?

Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?

07 August 2016 8,831 0 View

What causes overexpression of genes and how is it measured?

Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...

06 July 2016 3,993 9 View

Gene sequencing,multiple alignment and phylogenetic analysis

Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...

01 February 2016 2,846 4 View

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

01 February 2016 8,018 6 View

Protein expression and purification

Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.

01 February 2016 680 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View