How reliable are predicted sequences?

If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?

11 December 2017 5,640 0 View

Are humoral immune indexes such as lysozyme and antiproteases measured in terms of Opitcal density or Optical depth?

In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...

09 October 2016 8,552 2 View

Antiprotease activity, when a measure is negative, what could be the source of the problem?

My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...

08 September 2016 9,796 5 View

How long can sera samples from fish be stored and at what temperature?

I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.

08 September 2016 600 5 View

Why is the acute toxicity of many drugs measued using LD50?

07 August 2016 9,135 0 View

What causes overexpression of genes and how is it measured?

Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...

06 July 2016 3,993 9 View

Gene sequencing,multiple alignment and phylogenetic analysis

Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...

01 February 2016 2,846 4 View

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

01 February 2016 8,018 6 View

Protein expression and purification

Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.

01 February 2016 680 1 View

Gene cloning

How can one tell if a gene of interest has been previously cloned or not.

01 January 1970 386 9 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View