Dear colleagues, I would like a simple explanation as to why it is important to measure the toxicity of drugs using LD 50 and not anything below.
If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?
11 December 2017 5,778 0 View
In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...
09 October 2016 8,675 2 View
My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...
08 September 2016 9,923 5 View
I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.
08 September 2016 745 5 View
Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?
07 August 2016 8,965 0 View
Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...
06 July 2016 4,126 9 View
Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...
01 February 2016 2,984 4 View
I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?
01 February 2016 8,159 6 View
Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.
01 February 2016 831 1 View
How can one tell if a gene of interest has been previously cloned or not.
01 January 1970 517 9 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...
28 July 2024 4,168 0 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View