Dear colleagues, I would like a simple explanation as to why it is important to measure the toxicity of drugs using LD 50 and not anything below.
If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?
11 December 2017 5,640 0 View
In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...
09 October 2016 8,552 2 View
My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...
08 September 2016 9,796 5 View
I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.
08 September 2016 600 5 View
Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?
07 August 2016 8,831 0 View
Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...
06 July 2016 3,993 9 View
Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...
01 February 2016 2,846 4 View
I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?
01 February 2016 8,018 6 View
Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.
01 February 2016 680 1 View
How can one tell if a gene of interest has been previously cloned or not.
01 January 1970 386 9 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...
24 February 2021 1,534 2 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...
23 February 2021 9,159 3 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View