Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.
Just use any genome browser, I personally use the UCSC one, you will get the genomic, mRNA and protein sequence for the genes you look for.
If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?
11 December 2017 5,778 0 View
In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...
09 October 2016 8,675 2 View
My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...
08 September 2016 9,923 5 View
I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.
08 September 2016 745 5 View
Dear colleagues, I would like a simple explanation as to why it is important to measure the toxicity of drugs using LD 50 and not anything below.
07 August 2016 9,294 0 View
Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?
07 August 2016 8,965 0 View
Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...
06 July 2016 4,126 9 View
Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...
01 February 2016 2,984 4 View
I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?
01 February 2016 8,159 6 View
How can one tell if a gene of interest has been previously cloned or not.
01 January 1970 517 9 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View