Are humoral immune indexes such as lysozyme and antiproteases measured in terms of Opitcal density or Optical depth?

In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...

09 October 2016 8,552 2 View

Antiprotease activity, when a measure is negative, what could be the source of the problem?

My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...

08 September 2016 9,796 5 View

How long can sera samples from fish be stored and at what temperature?

I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.

08 September 2016 600 5 View

Why is the acute toxicity of many drugs measued using LD50?

07 August 2016 9,135 0 View

Which is the best time to take the weight of fish?

Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?

07 August 2016 8,831 0 View

What causes overexpression of genes and how is it measured?

Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...

06 July 2016 3,993 9 View

Gene sequencing,multiple alignment and phylogenetic analysis

Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...

01 February 2016 2,846 4 View

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

01 February 2016 8,018 6 View

Protein expression and purification

Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.

01 February 2016 680 1 View

Gene cloning

How can one tell if a gene of interest has been previously cloned or not.

01 January 1970 386 9 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

What does the value 0.498 in reliability?

There are 6 television programmes of different TV genres and their mean and mean ranks are put for each of them. But the reliability value of all the programmes together is 0.498 . So what exactly...

15 February 2021 7,686 4 View

Where can I find a reliable and updated methodology related with the design of spiral heat exchangers?

I need to design an spiral heat exchanger, buy I don't know what reference or bibliography (preferably a book) should I consult in order to get an updated and reliable methodology related with...

14 February 2021 7,910 3 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which kind of validity should e established in case we want to conduct a survey using newly developed survey questionnaire?

I am conducting a survey using self developed questionnaire. I have established its face validity and content validity. Some expert reviewer has suggested I must establish the validity by...

11 February 2021 2,077 3 View