Emmanuel Delwin Abarike

11 Questions 33 Answers 0 Followers

Questions related from Emmanuel Delwin Abarike

How reliable are predicted sequences?

If a gene of interest is not yet cloned but has a predicted sequence in NCBI, can one rely on this sequence to carry out gene expression studies?

12 December 2017 5,747 0 View

Are humoral immune indexes such as lysozyme and antiproteases measured in terms of Opitcal density or Optical depth?

In many article, authors use the abbreviation OD when describing protocols in measuring indexes such as; lysozyme, antiproteases, total protein among other humoral immune indexes. I understand...

10 October 2016 8,649 2 View

Antiprotease activity, when a measure is negative, what could be the source of the problem?

My measure of a control serum blank (trypsin without serum) antiprotease activity is lower than my tested samples. The percentage antiprotease activity therefore is negative when computed. What...

09 September 2016 9,894 5 View

How long can sera samples from fish be stored and at what temperature?

I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.

09 September 2016 696 5 View

Why is the acute toxicity of many drugs measued using LD50?

Dear colleagues, I would like a simple explanation as to why it is important to measure the toxicity of drugs using LD 50 and not anything below.

08 August 2016 9,234 0 View

Which is the best time to take the weight of fish?

Is it best to take the weight of fish after staving them for 24 hrs or for 12 hrs or otherwise?

08 August 2016 8,932 0 View

What causes overexpression of genes and how is it measured?

Often i see and hear researches explain gene expression as either up regulated or down regulated. if genes are said to be up regulated to what extend can it be considered hazardous, and how can...

07 July 2016 4,088 9 View

I want to wash my leucocytes cells with HBSS and adjust to 10 viable cells per mL.Could anyone explain to me how to practically do this?

02 February 2016 8,116 6 View

Protein expression and purification

Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.

02 February 2016 781 1 View

Gene sequencing,multiple alignment and phylogenetic analysis

Assuming your gene of interest has been sequenced and deposited at the gene bank and you want to carry out some other studies on this same gene, for example, to study gene expression following...

02 February 2016 2,939 4 View

Gene cloning

How can one tell if a gene of interest has been previously cloned or not.

01 January 1970 487 9 View