I have heard some researchers mentioned that sera samples if stored for more than 3 months could potentially affect some parameters. Does anyone have materials to support.
Hello Nirmala, the resources you provided are helpful. Thank you.
Storage depends on the nature of biochemical to be tested. Sera for enzymatic assays need storage at -80 for long time say 6 month to 1 year. But preferably enzymatic assays should be done from fresh samples. For steroids -20 degree storage yields good results upto 1 -1.5 yrs. Be sure not to freeze thaw them several times and keep them undisturbed until you proceed for the assay. The samples once taken out from -20 degree should be stored in crushed ice or mini cooler at 4 degree. Thaw only when you are about to proceed for the assays.
Regards
Srijit
Just a short remark about storage of fish sera. When we were testing sera of Cyprinid fish is was sufficient to keep them at -20C before specific antibody titration. However, in case of complement activity measurements it was required to keep the sera at -70 or -80C. Of course, it is advised to freeze only small amounts and thaw them only once just before testing.
It depends on the targeted compound to be tested. Generally it lay somewhere between -20 and -80 C for no more 1 year except few cases where storage could extend for few more months. However, it is preferable to perform measurements on fresh samples. Also, as mentioned in previous answers, try to avoid multiple freezing and thawing steps.
With best wishes SM Najim
- Badges
- Science topic
- Similar topics
- Biological Science
- Zoology
- Ichthyology
- Vertebrates
- Fish