Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can I ask did any good platform or journals for learning the calcluation on the reference governors and command governor?

Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...

23 February 2021 7,746 2 View

Does anyone know the nature of of Mycotoxin Immunoaffinity Columns contents?

Greetings I am working on the design of immunoaffinity columns for aflatoxin separation by HPLC method. Researchers who have experience in this field, can you please guide me regarding the...

10 October 2020 2,037 11 View

Can I use Ultrs AQ C18 column for aflatoxin ?

07 October 2020 9,811 2 View

Can we extract toxins from the media or the substrate it is growing in?

Actually these are two separate questions: i. How to extract mycotoxins from the media PDA the fungal culture is growing on? ii. Can we extract the toxin from the natural media it is growing on?

06 September 2020 8,193 5 View

The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis?

Dear Chromatographers, This method was developed on LC MS/MS. The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis. Any...

31 August 2020 1,216 9 View

In your opinion, Does Aflatoxin B1 cause a mutation in BARCA1gene and thus produce triple negative breast cancer?

In your opinion, Does Aflatoxin B1 cause a mutation in BARCA1 gene and thus produce triple negative breast cancer?

15 May 2020 608 52 View

What is the mechanism of nanomaterials to reduce or detoxification mycotoxins ?

I want to get a clear explanation that it is not adsorption or opening of the lactone ring, for example, nano-magnesium oxide, how it works when it binds or interacts with aflatoxin, what is the...

27 April 2020 2,972 4 View

How to calculate the amount of toxin per gram of wheat?

hello, I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat? the...

17 March 2020 7,469 3 View

Can we use freshly prepared mixture of aqueous solvents for extraction or can we make such mixture in bulk for later use?

For example, if we are using acetonitrile and water (84:16) mixture for the extraction of aflatoxin. So, whether this mixture (acetonitrile and water) is prepared fresh or not? and why?

14 March 2020 5,139 3 View

Non-consistent precursor ions in LC-MS/MS and reduced intensity after derivatization using TMS-DAM (peptide mass spec)?

Hi, I am trying to develop an assay to detect a conjugated peptide in plasma. I am essentially brand new to MS but have LC experience. The analyte of interest is a bisphosphonate tethered to...

12 January 2020 3,078 1 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View