Yes, it is possible to quantify mycotoxins, including aflatoxin B1 (AFB1), using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). LC-QTOF-MS is a powerful analytical technique that offers high sensitivity, selectivity, and accurate mass measurement, making it suitable for mycotoxin analysis in various food matrices, including cereals.

To quantify AFB1 using LC-QTOF-MS, you would typically follow a procedure that involves the following steps:

It's worth noting that LC-QTOF-MS can provide accurate mass measurement and high-resolution data, which can aid in the identification and confirmation of mycotoxins. However, the quantification of mycotoxins using LC-QTOF-MS requires careful method development and validation to ensure reliable and accurate results. Method validation includes assessing parameters such as linearity, sensitivity, precision, accuracy, and recovery.

It is recommended to consult relevant regulatory guidelines or standards for specific requirements regarding the quantification of mycotoxins, including AFB1, at mandatory levels in cereals. Additionally, working with an experienced analytical laboratory or expert in mycotoxin analysis can provide further guidance and support in developing and validating suitable methods for your specific needs.

Thank you Abdullah.

In fact, I am now going to try a more thorough extraction technique than the one I used before and also a cleanup step with SPE. As I don't fragment the ions (because the sensitivity is low) I use a mixture of internal standard 13C that helps to correct the matrix effect. So I'll cross my fingers.

Thanks again.