I have zero experience in GC-MS, but have to perform GC-MS to determine the toxin concentration in solution. Any advise would be helpfull. Thanks
Method:
1. Sample Preparation: Prepare the solution sample containing Aflatoxin B1. If necessary, concentrate or extract the sample to remove any interfering substances.
2. Cleanup: Perform a cleanup step to remove any additional impurities that might interfere with the analysis. Popular cleanup methods include solid-phase extraction (SPE) or immunoaffinity columns (IAC).
3. Derivatization (Optional): Aflatoxin B1 is not typically derivatized for GC-MS analysis, but if required for better detection or separation, derivatization methods such as silylation can be considered.
4. GC-MS Analysis: Inject the prepared sample into the GC-MS system.
Column Type:
For Aflatoxin B1 analysis, a capillary column with appropriate selectivity and efficiency should be chosen. The most commonly used column type for aflatoxins analysis is a polar or mid-polar phase column with low bleed characteristics. The column should have a high thermal stability to withstand the elevated temperatures required for GC analysis. A popular column choice for Aflatoxin B1 determination is a phenyl or cyanopropyl stationary phase column.
References:
1. "Determination of aflatoxins in food: performance criteria for analytical methods." European Commission. 2002. [Link](https://ec.europa.eu/food/system/files/2020-02/contaminants_catalogue_leg_reg_textlist_analytical_methods.pdf)
2. "Determination of aflatoxins in foodstuffs by GC-MS." FDA - Food and Drug Administration, Compliance Program Guidance Manual, Section 6830.001. August 2017. [Link](https://www.fda.gov/media/87546/download)
3. "Multiresidue method for determination of mycotoxins in cereals using gas chromatography-tandem mass spectrometry." Almeida, Adriana A., et al. Journal of AOAC International, vol. 97, no. 6, 2014, pp. 1690-1699. [Link](https://pubmed.ncbi.nlm.nih.gov/25876696/)
4. "Determination of aflatoxins in agricultural commodities by gas chromatography/mass spectrometry." AOAC Official Method 2000.08. AOAC International, Official Methods of Analysis, 17th ed. 2000. [Link](https://www.aoac.org/aoac_prod_imis/AOAC_Docs/StandardsDevelopment/OMA-gas-chromatography-mass-spectrometry)
These references will provide you with more detailed information on the analysis of Aflatoxin B1 using GC-MS and can serve as a starting point for method development and optimization.
- Badges
- Science topic