Tom20 antibodies for mice?

Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!

31 December 2019 1,162 1 View

Cell counting using flow cytometry?

All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?

11 December 2017 9,789 4 View

Cell viability using flow cytometry vs MTT?

All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?

10 November 2017 8,863 3 View

What is the best way to measure the activity of NOX2 using flow cytometry or biochemical method?

All - I saw different methods of measuring NOX2 activity using luciferin-based or NADPH reduction and etc.. What is really the best way of accessing this activity? Thanks!

10 November 2017 8,306 1 View

4 color imaging on confocal sp8?

Hello all- Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm. I want to use.. AF 488 AF 594 AF 647 I really want to avoid lower than 488...

05 June 2017 2,822 0 View

LC3 I/II and p62 weird ratio?

Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...

03 April 2017 9,122 2 View

ImageStream - Co-localization?

11 December 2016 8,546 0 View

FC block for intercellular staining for flow cytometry?

Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...

10 November 2016 6,752 4 View

96 wel culture-treated flat bottom plate - how to retrieve the cells?

09 October 2016 3,793 1 View

How can I make NEB Turbo Competent Cells in the lab?

Dear everyone- Has anyone tried to make NEB turbo competent cells in the lab? Is there any good protocol for this? Thank you so much for your time.

08 September 2016 9,716 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View