Tom20 antibodies for mice?

Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!

31 December 2019 1,162 1 View

FLT3L bone marrow dendritic cell - what is the best marker?

Hello, What would be the best marker for FLT3L-induced bone marrow dendritic cell for flow cytometry?

08 September 2018 6,653 0 View

Cell counting using flow cytometry?

All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?

11 December 2017 9,789 4 View

Cell viability using flow cytometry vs MTT?

All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?

10 November 2017 8,863 3 View

4 color imaging on confocal sp8?

Hello all- Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm. I want to use.. AF 488 AF 594 AF 647 I really want to avoid lower than 488...

05 June 2017 2,822 0 View

LC3 I/II and p62 weird ratio?

Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...

03 April 2017 9,122 2 View

ImageStream - Co-localization?

11 December 2016 8,546 0 View

FC block for intercellular staining for flow cytometry?

Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...

10 November 2016 6,752 4 View

96 wel culture-treated flat bottom plate - how to retrieve the cells?

09 October 2016 3,793 1 View

How can I make NEB Turbo Competent Cells in the lab?

Dear everyone- Has anyone tried to make NEB turbo competent cells in the lab? Is there any good protocol for this? Thank you so much for your time.

08 September 2016 9,716 0 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View