Tom20 antibodies for mice?

Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!

31 December 2019 1,162 1 View

FLT3L bone marrow dendritic cell - what is the best marker?

Hello, What would be the best marker for FLT3L-induced bone marrow dendritic cell for flow cytometry?

08 September 2018 6,653 0 View

Cell counting using flow cytometry?

All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?

11 December 2017 9,789 4 View

Cell viability using flow cytometry vs MTT?

All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?

10 November 2017 8,863 3 View

What is the best way to measure the activity of NOX2 using flow cytometry or biochemical method?

All - I saw different methods of measuring NOX2 activity using luciferin-based or NADPH reduction and etc.. What is really the best way of accessing this activity? Thanks!

10 November 2017 8,306 1 View

4 color imaging on confocal sp8?

Hello all- Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm. I want to use.. AF 488 AF 594 AF 647 I really want to avoid lower than 488...

05 June 2017 2,822 0 View

LC3 I/II and p62 weird ratio?

Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...

03 April 2017 9,122 2 View

ImageStream - Co-localization?

11 December 2016 8,546 0 View

FC block for intercellular staining for flow cytometry?

Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...

10 November 2016 6,752 4 View

96 wel culture-treated flat bottom plate - how to retrieve the cells?

09 October 2016 3,793 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View