15 Questions 8 Answers 0 Followers
Questions related from Woojong Lee
Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!
01 January 2020 1,225 1 View
Hello, What would be the best marker for FLT3L-induced bone marrow dendritic cell for flow cytometry?
09 September 2018 6,713 0 View
All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?
12 December 2017 9,854 4 View
All - I saw different methods of measuring NOX2 activity using luciferin-based or NADPH reduction and etc.. What is really the best way of accessing this activity? Thanks!
11 November 2017 8,360 1 View
All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?
11 November 2017 8,968 3 View
Hello all- Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm. I want to use.. AF 488 AF 594 AF 647 I really want to avoid lower than 488...
06 June 2017 2,888 0 View
Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...
04 April 2017 9,184 2 View
Hi Everyone, Has anyone analyzed colocalization using ImageStream? How reliable is this by itself without confocal (or any microscopic image)? What would be the potential disadvantages and...
12 December 2016 8,630 0 View
Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...
11 November 2016 6,834 4 View
Dear all- What would be the best way to retrieve the cells from 96 well culture-treated plates for staining and etc.? Thanks a lot!
10 October 2016 3,859 1 View
Dear everyone- Has anyone tried to make NEB turbo competent cells in the lab? Is there any good protocol for this? Thank you so much for your time.
09 September 2016 9,783 0 View
Hi all- I have a couple of questions about culturing DC 2.4 cell lines. I tried to find this from publications but I could not. Which media (DMEM vs. RPMI) is more ideal for DC 2.4 cells?...
08 August 2016 6,306 1 View
Hi all- What would be a good positive controls for phagocytosis/pinocytosis for color-based flow cytometry assay in antigen presenting cells?
08 August 2016 6,437 3 View
Dear all- What would be the best way to transfect macrophages/dendritic cells in vitro with plasmids for reporter assay or flourescent imaging with a good transfection efficiency without causing...
07 July 2016 3,522 9 View
Dear all I believe that it is okay to use cheap oligos (desalted) ones for screening, but is it absolutely required to use HPLC/PAGE-purified oligos? Has anyone succeeded in (or any...
01 January 2016 9,444 1 View
