Hello all-
Is there a way to image 4 colors on Leica SP8? We have UV (405 nm), and 45, 476, 488, 496, 514, 561, 633 nm.
I want to use..
AF 488
AF 594
AF 647
I really want to avoid lower than 488 as my cells give autoflouresence. Any thoughts?
Hello, Does anyone know tom20 antibodies that work well for mice? I tried Tom20 Antibody (F-10 and 29) and both did not work very well. Thanks!
31 December 2019 1,248 1 View
Hello, What would be the best marker for FLT3L-induced bone marrow dendritic cell for flow cytometry?
08 September 2018 6,740 0 View
All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?
11 December 2017 9,880 4 View
All- I was wondering if you can use cell viability test using live/dead for flow cytometry in lieu of MTT assay? Is this acceptable?
10 November 2017 9,002 3 View
All - I saw different methods of measuring NOX2 activity using luciferin-based or NADPH reduction and etc.. What is really the best way of accessing this activity? Thanks!
10 November 2017 8,384 1 View
Hello all, I treated with a type of adjuvant I am interested in with DC 2.4 cell lines (DC-like murine cells) for 3, 6, 12, and 24 hrs interval. I then lysed the whole cell extract and did WB...
03 April 2017 9,204 2 View
Hi Everyone, Has anyone analyzed colocalization using ImageStream? How reliable is this by itself without confocal (or any microscopic image)? What would be the potential disadvantages and...
11 December 2016 8,661 0 View
Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...
10 November 2016 6,859 4 View
Dear all- What would be the best way to retrieve the cells from 96 well culture-treated plates for staining and etc.? Thanks a lot!
09 October 2016 3,891 1 View
Dear everyone- Has anyone tried to make NEB turbo competent cells in the lab? Is there any good protocol for this? Thank you so much for your time.
08 September 2016 9,809 0 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View