What factors do you need before designing the exponential feeding in call culture? Also, what is the equation used in the exponential feeding? Experts in the group please comment. Thank you!!
Before designing exponential feeding in cell culture, there are several factors that need to be considered. These factors include:
The equation used for exponential feeding in cell culture is often derived based on the Monod equation, which describes the specific growth rate of cells as a function of substrate concentration. The specific growth rate (μ) can be expressed as:
μ = μmax * S / (Ks + S)
Where:
- μ is the specific growth rate of the cells.
- μmax is the maximum specific growth rate that can be achieved under optimal conditions.
- S is the substrate concentration.
- Ks is the saturation constant, representing the substrate concentration at which the growth rate is half of the maximum rate.
By controlling the substrate concentration (e.g., glucose) in the culture medium, the specific growth rate can be regulated to achieve exponential growth. The feeding rate can be adjusted based on the specific growth rate and the volume of the culture to maintain exponential feeding.
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