DNA extraction from human hair using QIAamp DNA Mini Kit?

Does anone have experience using the QIAamp DNA Mini Kit for the extraction of DNA from human hair? How much of starting material will be necessary. How is the pretreatment of the sample done...

25 October 2018 1,129 1 View

Are there any established parameters to evaluate signal quality of EMG?

20 April 2016 9,120 5 View

How can I improve reproducibility of the TUNEL assay in plants?

Dear all, is anyone of you experienced how to improve the reproducibility of TUNEL assay in plant/moss cell culture? I am trying to establish this assay to visualize DNA damages in nuclei. When I...

31 May 2015 7,727 3 View

How can I isolate protein from fat tissue?

I am trying to isolate membrane bound protein from a number of different mouse tissues and I wanted to know if high salt (400mM) RIPA buffer would work on fat.

10 June 2014 3,564 3 View

How can I stain primary B cells (mouse) with an antibody against mouse without having all their antibodies detected by the anti mouse sec. ab in IF?

I already tried blocking, which was fine for lines, but the B cells present so many antibodies that I cannot cover. The secondary antibody control still shows massive cytoplasmic/membrane...

20 February 2013 2,989 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View